Abstract
In this study, the kinetic isotope effects and solvent isotope effects in the reaction of the deamination of [(1R)-2H ] putrescine – catalysed by enzyme diamine oxidase (EC 1.4.3.6) – were determined using a non-competitive spectroscopic method. Putrescine, stereospecifically labelled with deuterium, was obtained by enzymatic decarboxylation of l-ornithine that was carried out in a fully deuteriated incubation medium.
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