Kinetic analysis of the chlorophyll fluorescence inductions from chloroplasts blocked with 3-(3,4-dichlorophenyl)-1,1-dimethylurea

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1. The induction of Photosystem II chlorophyll fluorescence from chloroplasts blocked with 3-(3,4-dichlorophenyl)-1,1-dimethylurea and uncoupled with gramicidin has been measured. 2. In agreement with other authors it was found that the addition of cations to chloroplasts suspended in a low-cation medium not only stimulated the intensity of fluorescence but also changed the shape of the induction from being nearly exponential to being sigmoid. 3. A new theory of the photosynthetic unit of Photosystem II (Paillotin, G. (1976) J. Theor. Biol. 58, 237–252) was used to analyse the fluorescence inductions. 4. A comparison of the results of the Paillotin model with the experimental data suggests that excitation energy is not able to migrate between all the photosynthetic units of a photosynthetic domain. However, it is concluded that excitation energy may migrate from one photosynthetic unit to another, and that the energy migration is in competition with other processes leading to the decay of the excitation within Photosystem II. 5. It is suggested that the size of the “functional” photosynthetic unit, defined as the number of chlorophyll molecules that may communicate with a reaction centre, is variable.