Kinetic analysis of hemoglobin detergency by probability density functional method.

Miyako Oya,Naoaki Fujimura,Yosuke Taniguchi,Karen Miyamoto,Masaru Oya,Anjani Kumar Tiwari

doi:10.1371/journal.pone.0237255

Miyako Oya, Naoaki Fujimura + Show 4 more

Open Access

https://doi.org/10.1371/journal.pone.0237255

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Journal: PloS one	Publication Date: Aug 7, 2020
Citations: 10	License type: CC BY 4.0

Affiliation: Kin-ikyo Chuo Hospital, Yokohama National University

Abstract

In this study, washing tests were performed using samples prepared by contaminating fabrics with hemoglobin, and a kinetic analysis was conducted based the probability density functional method, which expresses the cleaning power using two parameters σrl (related to the cleaning mechanism) and μrl (related to the level of cleaning power). This method allows for the processing of uncertainties specific to protein washing under the assumption that the soil adhesion and detergency are in accordance with a normal distribution. A certain amount of hemoglobin solution was soaked in a cloth, dried, and steam-treated, and then used as a sample for a cleaning test. Two parameters σrl and μrl were calculated based on the detergency (%) after 5 min, 10 min, 15 min, and 20 min of washing with respect to different pH and temperature levels, and different sodium dodecyl sulfate (SDS) concentration and temperature levels. Based on the results, the value of σrl indicated that the hemoglobin was removed by the dissolving action. In addition, μrl increased in accordance with an increase in the pH, SDS concentration, and temperature. With respect to μrl, the relationship of ΔX + ΔY = Δ(X+Y) was observed in several cases, where ΔX represents the effect of the pH or SDS concentration, ΔY is the temperature effect, and Δ(X+Y) is the combined effect. Therefore, there may be an additive relationship between the pH and temperature effects, and the SDS concentration and temperature effects.

Highlights

In the medical field, with respect to hygiene, the cleaning of blood stains adhered to medical devices and surgical material is critical
The time-dependent curves of the detergency obtained by varying the pH in steps of 6, 9, 10, and 11 at cleaning temperatures of 30 ̊C, 40 ̊C, 50 ̊C, and 60 ̊C, respectively, are shown in Fig 2.Since it was expected that the cleaning efficiency would be low when the pH was low at low temperature and the cleaning effect due to the temperature and pH increase would be difficult to appear, the minimum temperature was set to 30 ̊C
Based on the application of the probability density functional method to the analysis of the detergency of hemoglobin stains, additive effects were observed between the pH and temperature effects, and between the sodium dodecyl sulfate (SDS) concentration and temperature effects, under multiple conditions

Summary

Introduction

With respect to hygiene, the cleaning of blood stains adhered to medical devices and surgical material is critical. In several studies, the effectiveness of automatic cleaning was compared with that of manual cleaning for the cleaning of medical instruments such as special cutting instruments, forceps, and endoscopes [1,2,3,4]. Studies were conducted on the effects of cleaning agents and disinfectants on the cleaning of medical devices and materials [5,6,7]. Adenosine triphosphate (ATP) tests were conducted, and alternative practical detergency evaluation methods were implemented [8,9,10,11,12,13], which highlighted the importance of monitoring [14,15,16]. Several fundamental studies were conducted with focus on the interaction of blood proteins such as albumin and hemoglobin with surfactants [17,18,19]

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Results

Conclusion