Abstract
Currently used methods for investigating kinetics of peptide degradation such as refractive index monitoring, radioimmunoassay (RIA), high-performance liquid chromatography (HPLC), or capillary electrophoresis (CE) are time consuming, need large amounts of substrate, and are often too insensitive. Moreover, as in the case of RIA, HPLC, and CE, it is often impossible to interpret the observed results with confidence in the integrity of the analyte. To circumvent such obstacles, we found matrix-assisted laser desorption/ionization (MALDI) used with time-of-flight mass spectrometry (TOFMS) not only useful for qualitative analysis of reaction pathways but also for quantification. In the following chapter, we give two examples of kinetic reaction course evaluation, one non-enzymatic and one enzymatic.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
