Abstract
The use of biomass of microalgae as a feedstock to produce bioethanol is very promising, it is caused by a large amount of carbohydrates contained in microalgae physiology cell. The main obstacle of enzymatic hydrolysis in order to produce bioethanol is the bound starch granules in a rigid cell wall. Therefore, pre-treatment steps needed to remove and convert complex carbohydrates into simple sugars before the fermentation process. Tetraselmis Chuii microalgae species are green microalgae (Chlorophyta) in which the cell wall containing cellulose and hemicellulose as the main constituent, therefore, this study observe the effect of the use of cellulase enzymes and xylanase as a strategy to open up the cell walls of microalgae. Another investigated parameter is the enzyme concentration, temperature, pH, and methods of use of enzymes. The results showed that the highest yield of glucose obtained was 31.912% (w / w) and is achieved under the conditions of a temperature of 45oC, pH of 4.5, the amount of biomass of microalgae as 5 g/L, the concentration of cellulase enzymes and xilanase 30% (w / w) at 40 minute at mechanism using cellulase and xylanase enzymes simultaneously.
Highlights
The use of biomass of microalgae as a feedstock to produce bioethanol is very promising, it is caused by a large amount of carbohydrates contained in microalgae physiology cell
The results showed that the highest yield of glucose obtained was 31.912% (w / w) and is achieved under the conditions of a temperature of 45oC, pH of 4.5, the amount of biomass of microalgae as 5 g/L, the concentration of cellulase enzymes and xilanase 30% (w / w) at 40 minute at mechanism using cellulase and xylanase enzymes simultaneously
R., Michael, K. and Danquah, Enzymatic hydrolysis of microalgal biomass for bioethanol production, Chemical
Summary
Sejarah Artikel: Diterima Oktober 2016 Disetujui November 2016 Dipublikasikan Desember 2016. Penggunaan biomassa mikroalga sebagai bahan baku untuk memproduksi bioetanol sangat menjanjikan, hal ini disebabkan oleh sejumlah besar karbohidrat terdapat dalam fisiologi sel mikroalga. Kendala utama dari hidrolisis enzimatik dalam rangka memproduksi bioetanol adalah terikatnya granula pati dalam dinding sel yang kaku. Mikroalga spesies Tetraselmis chuii merupakan mikroalga hijau (Chlorophyta) yang mana dinding selnya mengandung selulosa dan hemiselulosa sebagai konstituen utama, oleh karena itu, penelitian ini melihat pengaruh penggunaan enzim selulase dan xilanase sebagai strategi untuk membuka dinding sel mikroalga. Parameter yang berbeda yang akan diselidiki adalah konsentrasi enzim, suhu, pH, serta metode penggunaan enzim. Hasil penelitian menunjukkan bahwa yield tertinggi glukosa yang diperoleh adalah 31,912% (w/w) dan ini dicapai pada kondisi proses suhu 450C, pH 4,5, jumlah biomassa mikroalga 5 g/L, konsentrasi enzim selulase dan xilanase masing-masing 30% (w/w) pada waktu 40 menit pada mekanisme penggunaan enzim selulase dan xilanase secara simultan
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
