Abstract
The study aimed to examine the kinematics motility and recovery rates of frozen-thawed Friesian Holstein bull sexing semen in the addition of l-ascorbic acid treatment. Semen collected from Friesian Holstein bulls with the minimum standard of sperm motilities for sexing sperm (60%), and abnormality (<20%) used for this study. Semen was diluted with final concentration 300x106 sperm/ml in Brackett-Oliphant (BO) medium. The sperms were sorted using the BSA column method. Sexing sperm were extended with Tris egg yolk (TEY) extender in addition L-Ascorbic Acid (LA) 0%, 0.25%, 0.5%, and 0.75% before freezing process. Frozen sexing semen was thawed for the kinematics motility evaluation with parameter; Tmot, Pmot, VAP, VCL, VSL, LIN, ALH, BCF, DAP, DCL and DAP using CASA system also recovery-rate evaluation. The results showed kinematics motility evaluation for 0.75% TEY-LA had lowest value (P<0.05) in Tmot, Pmot, velocity and distance compared to control group, 0.25% and 0.5% TEY-LA. The recovery rate 0.5% TEY-LA had highest values between among all treatment in X and Y frozen sexing semen. In conclusion, the addition of 0.5% l-ascorbic acid to tris egg yolk extender could be advantageous tool to improve the kinematics motility and increased recovery rates of Friesian Holstein sexing sperm.
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