Kindling epilepsy models with kainic acid in different parts of rat brain

Abstract

Objective To discussion the methods of simpler production and better effect in the kainic acid induced epilepsy model. Methods By stereotactic operation,injection the kainic acid 1.0 μl to the insular, the amygdala and the lateral ventricle of the rats, observing the changes of the behavior, the EEG and the pathology and comparing the ignition characteristic in epileptic rats. Results The change of the behavior, the EEG and the pathology in epileptic rats confirmed that the rats of injection the kainic acid to insular can be successfully established experimental animal models. Compared to the traditional position (injection the kainic acid to amygdala and lateral ventricle) ,the insular ignite group primary seizure time is (59. 96 ±4. 36) min,static duration is(11. 90 ±0. 54) d,significantly faster than the amygdala group and the lateral ventricle group ( P ＜ 0. 05 ). Conclusion The insular has the seizure potency, the kainic acid injection the rat' s insular has the advantages in shortened test cycle and reduced the cost of experiments. Key words: Kainic acid; Epilepsy ; Insular; Amygdala