Abstract
This study aims to compare the efficacy of different treatments for nude mice transplanted with HT-29 colon carcinoma cell line. BalB/C nude mice were transplanted with HT-29 colon carcinoma cell line and randomly divided into four groups, with 5 mice in each group: blank control group, DC-CIK group, Huaier Granule group, and Huaier Granule group combined with DC-CIK group (combined treatment group). For DC-CIK group and combined treatment group, 1×106 DC-CIK cells were injected via the tail vein 4 days after transplantation. The injection was performed twice weekly for a total of 2 weeks. For Huaier Granule group and combined treatment group, Huaier Granule was administered at the dose of 20 g/60 g, by dissolving 20 g of Huaier granules in 600 ml of pure water. Intragastric administration of 0.2 ml of granules was performed once daily for 3 weeks. For the blank control group, equal volume of normal saline was given. Tumor size and body weight of nude mice were measured every 2 days during the 3-week treatment. The mice were sacrificed at the end of treatment to harvest tumors. Key genes of the signaling pathway were detected by RT-PCR. At the end of treatment, mice in combined treatment group, DC-CIK group and Huaier Granule group remained stable emotionally with normal mobility and water and food intake. However, in the blank control group, the mobility was restricted starting from the third week and the mice were on the verge of dying. The expression of PI3KR1, Akt, Wnt1, CTTNB1, Notch1, Notch2 and Notch3 genes were all downregulated significantly in the combined treatment group compared with DC-CIK group and Huaier Granule group (P<0.05). Therefore, the combined treatment of Huaier Granule combined with DC-CIK achieved the best effect in nude mice transplanted with HT-29 colon carcinoma cell line.
Highlights
Colon carcinoma is a common gastrointestinal cancer in clinic
This study aims to compare the efficacy of different treatments for nude mice transplanted with HT-29 colon carcinoma cell line
BalB/C nude mice were transplanted with HT-29 colon carcinoma cell line and randomly divided into four groups, with 5 mice in each group: blank control group, Dendritic cells (DCs)-CIK group, Huaier Granule group, and Huaier Granule group combined with DC-CIK group
Summary
Colon carcinoma is a common gastrointestinal cancer in clinic. In China, it ranks the 3rd and 5th position in terms of incidence and mortality, respectively. China has witnessed an increasing prevalence of colon carcinoma since 1980s, by 38.56% in 15 years [1]. Because of atypical early symptoms, colon carcinoma is usually associated with a high misdiagnosis rate, high malignancy, delayed diagnosis and easy metastasis. Constant efforts are made to find out the reason for the low 5-year survival rate in colon carcinoma. Dendritic cells (DCs) are the most powerful antigen-presenting cells that can activate initial T cells and specific immune response and facilitate nonspecific cytotoxic killing. They are important antigen carriers in anti-tumor immunity. With rapid in vitro proliferation and broad-spectrum killing effects, CIK cells are sensitive to drug-resistant tumor cells and considered ideal immunocompetent cells
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