Killing effect of cytokine-induced killer cells on human lung adenocarcinoma and its radiation resistant cell lines

Abstract

Objective To study the killing effect of cytokine-induced killer cells (CIK cells) on human lung adenocarcinoma cell line (A549) and the lung adenocarcinoma's radiation resistant cell line(A549RR). Methods Peripheral blood mononuclear cells (PBMC) of healthy volunteers were stimulated by different cytokines, and were induced into killer activity CIK cells. The phenotype of CIK cells were analyzed by flow cytometer. A549 and A549RR cell lines were cultured separately with the CIK cells. The absorbance value (A) of the cells was measured by CCK8, and the killing rates of all cells which were cultured for 24 and 48 hours with the CIK were calculated. Results The rate of CD3+ CD56+ cell was 45.8 % after culture for 14 d. The killing rates of CIK cells to lung adenocarcinoma A549 cells and its radiation resistant cells A549RR were increased with the rise of the ratio of effective cells to target (5:1-40:1) and the increasing of culturing time (all P 0.05). Conclusion CIK cells have strong anti-tumor effect against lung adenocarcinoma and its radiation resistant cells with high clinical application value. Key words: Cytokine-induced killer cells; Lung adenocarcinoma; Radiation resistant