Abstract
Objective: This study used Ki-67 to evaluate the proliferative activity of ameloblastoma in an attempt to provide a scientific basis for any differences in the biologic behaviour that exists between the different types of ameloblastoma and correlate the results with the recurrence rate. Study design: We assessed Ki-67 expression by immunohistochemistry in 32 cases of mandibular ameloblastoma. Formalin-fixed, paraffinembedded blocks were sectioned and used for Haematoxylin & Eosin (H&E) staining. H&E slides were used to re-evaluate the histological types of the ameloblastoma. Immunohistochemical analysis for Ki-67 with a labelled streptavidin-biotin-peroxidase complex technique was performed on tumour sections using Monoclonal Mouse Anti-Human Ki-67 Antigen (clones MIB1, code M7240, Dako Corporation). Lymph nodes (lymphoma) were used as positive control for Ki-67. Results: The specimens comprised 18 cases of Follicular ameloblastoma, 7 cases of Cystic ameloblastoma, 4 cases of plexiform ameloblastoma, and 3 cases of Acanthotic ameloblastoma. The results showed that cellular proliferative activity varied within the ameloblastoma types. There was no statistically significant difference in the Ki-67 Acanthotic, cystic and follicular types of ameloblastoma, while plexiform type showed statistically significantly higher levels than the other 3 types. The mean Ki-67 labelling index of plexiform ameloblastoma ranged from 13 to 30 with a mean of 19.75. The mean values of Ki-67 were statistically significantly higher in recurrent than non-recurrent cases. Conclusion: The significant expression of Ki-67 could provide useful prognostic markers for proliferative activity and good prognostic indicators for recurrence rate of mandibular ameloblastoma.
Highlights
Ameloblastoma is a Benign Tumour of Odontogenic epithelium with mature fibrous stroma without odontogenic ectomesenchyme
The results showed that cellular proliferative activity varied within the ameloblastoma types
The aim of the present study is to evaluate the Ki-67 labelled index in different histologic types of mandibular ameloblastoma and their relation to proliferative activity and local recurrence
Summary
Ameloblastoma is a Benign Tumour of Odontogenic epithelium with mature fibrous stroma without odontogenic ectomesenchyme. Correlation between these histological patterns and tumour behaviour has not been consistently established [2]. Determination of epithelial proliferative activity is apotentially useful means of investigating differences inbiologic behaviour between tumours [5,6,7]. A. Hegab et al / Open Journal of Stomatology 3 (2013) 520-526 of cell proliferation in many types of tumours is an importent adjunct to histologically based tumour classification and has potential relevance as an indicator of tumour-behaviour and treatment response and relapse. The interpretation, merits, and clinical value of the methods of proliferation assessment in tumours are detailed by van Diest et al [9]. The aim of the present study is to evaluate the Ki-67 labelled index in different histologic types of mandibular ameloblastoma and their relation to proliferative activity and local recurrence
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