(Keynote, Digital Presentation) A Study of Nanozyme-Based Biosensor

Abstract

Nanozymes have attracted significant research interest over the world due to their simple preparation, storage, as well as the low-cost compared with natural enzymes. We started to study nanozymes over 10 years ago [1-8]. A few examples of novel nanozymes from our laboratory are shown: 1, NiPd hNPs exhibited triple-enzyme mimetic activities (oxidase-like, peroxidase-like and catalase-like activities). 2, Fe3O4 NPs on 3D porous graphene exhibited enhanced nanozyme activity and used for glucose determination. 3, GOx@ZIF-8(NiPd) nanoflower exhibiting tandem catalysis has been firstly proposed.Recently, a new nanozyme based on a bionic zeolitic imidazolate framework-8 (ZIF-8) has the active center similar to hCAII, showing hCA as well as esterase and acetylcholinesterase-like activities. A new class of single-atom nanozymes, as FeN5 SA/CNF, with atomically dispersed enzyme-like active sites in nanomaterials has been discovered. The defined single-atom nanozymes provide a new perspective to the catalytic mechanism and rational design of nanozymes and exhibit great potential to become the next-generation nanozymes. References H. Wei, E. K. Wang, Chem. Soc. Rev., 2013, 42, 6060-6093. H. Wei, E. K. Wang, Anal. Chem., 2008, 80, 2250-2254. Q. Wang, X. Zhang, L. Huang, Z. Zhang, S. J. Dong, Angew. Chem. Int. Ed., 2017, 56, 16082. S. L. Rong, Y. C. Huang, J. W. Liu, E. K. Wang, H. Wei, Prog. Biochem. Biophys. 2018, 45, 129-147. Q. Wang, H. Wei, Z. Zhang, E. K. Wang, S. J. Dong, Trends Anal. Chem., 2018, 105, 218-224. WW. Wu, L. Huang, EK. Wang, SJ. Dong, Chem. Sci. 2020 11, 9741-9756. J. X. Chen, L. Huang, Q. Q. Wang, W. W. Wu, H. Zhang, Y. X. Fang, S. J. Dong, Nanoscale, 2019, 11, 5960-5966. L. Huang, J. X. Chen, L. F. Gan, J. Wang, S. J. Dong , Sci. Adv. 2019, 5, eaav5490. Acknowledgment This work was supported by the National Natural Science Foundation of China and The Ministry of Science and Technology of ChinaAll coworkers in this laboratory are appreciated for their effort in this area Key words Nanozymes, NiPd NPs, GOx@ZIF-8(NiPd) nanoflower, ZIF-8, acetylcholinesterase-like nanozyme , single-atom nanozyme- FeN5 SA/CNF