Key genes for thrombi resolution in a rat model of traumatic deep vein thrombosis

Abstract

Objective To screen the key genes for thrombi resolution in a rat model of trauma deep vein thrombosis (DVT). Methods Seventy Sprague Dawley rats were divided into a normal control group (group A,10 rats) and a model group (60 rats).The traumatic DVT model was established by quantitative beating on the bilateral posterior limbs.From the model group a thrombosis group (group B,10 rats) and a thrombosis-resolution group (group C,10 rats) were screened.The Genechip Rat genome 430 2.0 was used to detect changes in gene expressions on difference phases of DVT after trauma.Time series analysis was used to screen the key genes that affected the phenotype changes associated with thrombi resolution. Results COL11-α1,GUS-β,MMP12,FAP,C1QTNF3_PREDICTED,ACP-5,TBC1D12_PREDICTED and CAPN-6 were screened as the key genes for thrombi resolution in traumatic DVT.In groups A,B and C,the abundance expressions of COL11-α1 were respectively 0,2.5 and 1.2; those of GUS-β were respectively 0,1.5 and 0.7; those of MMP-12 were respectively 0,3.9 and 1.6; those of FAP were respectively 0,1.8 and 0.5; those of C1QTNF3_PREDICTED were respectively 0,5.7 and 3.5; those of ACP-5 were respectively 0,1.2 and 0.8; those of TBC1D12_PREDICTED were respectively 0,1.7 and 0.8.; those of CAPN-6 were respectively 0,4.5 and 2.9.These genes had the same expression mode and coincided with the biological phenotype of thrombi. Conclusion In the process of traumatic DVT,as COL11-α1,GUS-β,MMP-12,FAP,C1QTNF3_PREDICTED,ACP-5,TBC1D12_PREDICTED and CAPN-6,have an intimate relation with the resolution of thrombi,they may be the key genes that promote thrombi resolution. Key words: Wounds and injuries; Venous thrombosis; Genes; Oligonucleotide array sequence analysis