Abstract
What happens when protozoa are injected onto an HPLC column? Because they do not have a rigid cell wall, they instantaneously release their contents, which can thus be analyzed without loss or opportunity for artefact formation (“ex vivo HPLC”). This method applied to the example, Pseudokeronopsis rubra (picture below), showed that the organism stores 1 in the form of the sulfate ester (R1 = SO3Na), which only on destruction of the cells is converted enzymatically into the more toxic pyrone 1 (R1 = H), R2 = H, Br.
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