Keratinolytic Protease Production by Bacillus Cereus Strain Ps03 under Submerged Fermentation: Optimization and Characterization

M.D Balakumaran,R Santhi

doi:10.3126/ijasbt.v4i3.15780

Abstract

In the present study, chicken feather powder was screened for its application as the substrate for the production of keratinolytic protease by Bacillus subtilis strain PS03. Bacillus subtilis produced a high level of keratinolytic protease using chicken feather powder as substrate. With feather powder as substrate, physical factors such as incubation time, pH and temperature were optimized for increased keratinolytic protease production by Bacillus subtilis. The enzyme production was enhanced when using maltose as carbon source and yeast extract as nitrogen sources. SDS-PAGE analysis indicated the molecular weight of 46 kDa of the partially purified keratinolytic protease. The keratinolytic protease enzyme was stable over a pH range of 6 – 9 and temperature range of 35 - 50°C with maximum activity at pH 9 and 40°C. Based on the results, the use of feather powder as substrate for keratinolytic protease production is cost effective and is easy to scale up. Considering the availability and cost, chicken feather powder is considered as an ideal substrate for keratinolytic protease production in an industrial point of view. Int J Appl Sci Biotechnol, Vol 4(3): 397-401

Highlights

Microorganisms serve as the production warehouse for many industrially important enzymes (Mitidieri et al, 2006)
The present study aimed to evaluate the keratinolytic enzyme production by Bacillus subtilis PS03 in submerged fermentation, its optimization and characterization
Bacterial Strain The proteolytic bacteria, Bacillus subtilis strain PS03 isolated from tannery effluent contaminated soil was used for the present investigation

Summary

Introduction

Microorganisms serve as the production warehouse for many industrially important enzymes (Mitidieri et al, 2006). Keratin can be degraded by keratinolytic enzymes produced by certain species of the genus Bacillus (Cedrola et al, 2012) and fungi (Gradisar et al, 2005). The keratinolytic proteases play an important role in the enzymatic production of feather meal and feed additives for cattle (Odetallah et al, 2005), amino acid production, leather and detergent industries, etc. Bacillus subtilis is one of the most important industrial bacterial species that produces a variety of enzymes such as amylases, cellulase and proteases (Morya et al, 2012). Have been used in food industries for several decades Even though their proteases have been studied, reports on keratinolytic protease production by Bacillus subtilis are not in-depth (Lopes et al, 2011). The present study aimed to evaluate the keratinolytic enzyme production by Bacillus subtilis PS03 in submerged fermentation, its optimization and characterization

Objectives

Methods

Results

Conclusion