Kemampuan Kitinase Streptomyces RKt5 sebagai Antijamur terhadap Patogen Fusarium oxysporum

Yurnaliza Yurnaliza,Langkah Sembiring,Sebastian Margino

doi:10.31258/jnat.14.1.42-46

Yurnaliza Yurnaliza, Langkah Sembiring + Show 1 more

Open Access

https://doi.org/10.31258/jnat.14.1.42-46

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Abstract

The purpose of the reasearch is to determine of antifungal activity from chitinase from Streptomyces RKt5 to inhibite growth of Fusariumoxysporum. The chitinase of Streptomyces RKt5 produced in liquid chitin medium with optimum conditions (inoculum concentration, pHand incubation time) and then partially purified with ammonium sulphate. The enzyme products were tested the antifungal activity againstF.oxysporum. The results showed that mycelial growth of F.oxysporum can be inhibited by Streptomyces RKt 5 in dual culture test. Thepartial purified chitinase enzyme couldn’t inhibit the fungal growth. But if the mycellium fragmented, the enzyme could degrade the fungalcell wall in incubation time. The frequency of fungal cell wall lysis and levels of N-acetylglucosamine released that have been increasingalong with the length of incubation time.

Highlights

PENDAHULUAN Kitinase merupakan enzim hidrolitik yang dapat mendegradasi kitin yaitu polimer dari b-1,4 N-setil-Dglukosamin
The results showed that mycelial growth of F.oxysporum can be inhibited by Streptomyces RKt 5 in dual culture test
Kemampuan antijamur dari kitinase Streptomyces RKt5 akan efektif jika mikrobia kitinolitik langsung berinteraksi dengan jamur patogen pada keadaan terinduksi kitin

Summary

Introduction

PENDAHULUAN Kitinase merupakan enzim hidrolitik yang dapat mendegradasi kitin yaitu polimer dari b-1,4 N-setil-Dglukosamin. Maka pada penelitian ini akan dikaji kemampuan Streptomyces RKt5 sebagai antijamur terutama dalam melisiskan dinding sel jamur Fusarium oxysporum. Uji antagonis dilakukan untuk melihat kemampuan bakteri Streptomyces RKt5 menghambat pertumbuhan jamur F.oxysporum pada kondisi terinduksi kitin. Pada pinggir miselium jamur umur 3 hari di medium PDA, diletakkan cakram kertas yang telah direndam cairan enzim hasil purifikasi (konsentrasi 8,92 mg prot/ml) dengan jarak 1 cm dari pinggir koloni jamur.

Results

Conclusion