Abstract
In this study, useful lactic acid bacteria were isolated from kefir, which contains complexes of bacteria, yeast and serves as a reservoir for beneficial strains. As a result of 16s rRNA sequence analysis of 2 strains of kefir-derived lactic acid bacteria with remarkable antimicrobial activity, both BMSE-K006 and K009 showed 99.86% homology with Lactobacillus pentosus DSM 20314. The availability of carbohydrate fermentation patterns of L. pentosus BMSE-K006 and K009 were similar, and the availability of D-galactose and lactose was observed, confirming the availability of sugar in milk or dairy products. Also the antimicrobial activity of L. pentosus BMSE-K006 and K009 showed the highest against pathogenic bacteria such as Staphylococcus spp., Escherichia spp., Pseudomonas aeruginosa, but no antifungal effect was observed against Candida albicans strains. L. pentosus BMSE-K006 and K009 cultured supernatant showed DPPH radical scavenging activity of 69.02% and 65.88%, respectively. Therefore, these results are expected that L. pentosus BMSE-K006 and K009 could be potential resources as useful lactic acid bacteria isolated from kefir.
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