Keeping plant tissue slices attached to the slide during harsh extraction of polysaccharide.

Abstract

During extraction of hemicellulose and noncellulose polysaccharides from plant histological slices of plants the morphological integrity of the slices may be disturbed if the reagents extracting hemicellulose and noncellulose polysaccharides are rinsed away by water. Removing the reagents by delicate suction rather than removing the glass tray from the staining dish, followed by desiccation and a rinse in 95% ethanol terminates the treatment without altering the morphology of the tissues.