Abstract
Production of waste in urban areas reached 0.5 kg/person /day and 80% consisted of organic waste, one of many types of waste mostly generated is household waste. Generally, this household organic waste containing structural compoundsuch us long chains of cellulose.Therefore, the use of microorganism that can produce cellulase is very important to reduce the weight of garbage. Trichoderma harzianumis known as the most potential mold compared to other molds in converting cellulose. Sawdust and bran contain cellulose and hemicellulose that can be used as the main component in the mediafor its growth. The objectives of this study was: To find an effective ratio between sawdust and bran as growth mediafor Trichoderma harzianum, To know the cellulase activity of Trichoderma harzianum. This study has been conducted from April 2015 to Juli 2015 in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Andalas. The results of this study concluded that an effective ratio between sawdust and bran as a growth media for Trichoderma harzianumwhich degraded organic waste was 50:50. The highest activity of cellulase in degrading organic waste is 100%.
Highlights
80% consisted of organic waste
important to reduce the weight of garbage
most potential mold compared to other molds in converting cellulose
Summary
KECEPATAN PERTUMBUHAN KAPANG (Trichoderma harzianum Rifai A1300-F006) DAN AKTIVITAS SELULASE DALAM PENANGANANAN SAMPAH SELULOSA. Pebriana Nasution*, Periadnadi, Nurmiati Laboratorium Mikrobiologi, Jurusan Biologi, FMIPA Universitas Andalas, Limau Manis, Padang 25163. Trichoderma harzianum dikenal sebagai kapang paling potensial dibandingkan kapang yang lainnya dalam mengkonversi selulosa. Serbuk gergaji dan dedak memiliki kandungan selulosa dan hemiselulosa yang dapat digunakan kapang sebagai komponen utama pertumbuhannya. Tujuan penelitian ini untuk mengetahui perbandingan efektif antara serbuk gergaji dan dedak sebagai media pertumbuahan kapang T. harzianum dan untuk mengetahui aktifitas enzim selulase kapang T. harzianum. Penelitian ini dilaksanakan pada bulan April 2015 sampai Juli 2015 di Laboratorium Mikrobiologi, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas. Dari hasil penelitian ini disimpulkan bahwa perbandingan efektif antara serbuk gergaji dan dedak sebagai media pertumbuhan kapang pendegradasi sampah organik adalah 50:50, Aktifitas enzim selulase tertinggi terdapat pada konsentrasi 100%.
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