Abstract
BackgroundCancer cells, even in the presence of available oxygen, have a glycolysis enhancement. The “aerobic glycolysis” is known as the Warburg effect and it is considered one of the fundamental hallmarks of metabolic alteration during malignant transformation. A feature of many tumors is also a change into ions equilibrium, with particular reference to K+ intracellular concentration. Another hallmark in cancer is the reprogrammed chemotaxis pathways in favour of tumor cell dissemination.ResultsThe doubling population time of 5 mM K:D-rib treated Hs 578T (HTB-126 ® ATCC) cell line is reduce by 30% respect to the control. During the chemotactic invasion assay, the relative number of motile and invasive cells, counted inside the FBS-AGAR spot, shows a decrease with the maintenance of the treatment reaching the 25% after nine days. Hs 578Bst (HTB-125 ® ATCC) non-tumor cell line treated for nineteen days with 5 mM K:D-rib was split twice as well as the control. No morphological change was visible in the treated respect to untreated cells.ConclusionsWe demonstrate that the synergic action of potassium bicarbonate and D-ribose has effect on Hs 578T cancer cell line proliferation reducing the cell cycle time. At 5 mM concentration, K:D-rib is able to modify the tumorigenic potential of human breast cancer cell line Hs 578T, interfering in vitro with the capability of Hs 578 T cell line to migrate under chemotactic stimuli. Despite this, K:D-rib solution, does not exhibit any appreciable toxicity as confirmed by the proliferation assay accomplished on Hs 578Bst cell line.
Highlights
In this paper we investigate the effects of potassium hydrogen carbonate and D-ribose in water solution (K:D-rib) on the human breast cancer cell line - Hs 578T
Our previous study [1] focused on the strong antioxidant effect of potassium ascorbate on red blood cell oxidation [2,3] highlighted the ascorbate involvement as carrier of K+ inside the cells
Cells and culture conditions Hs 578T adherent human breast cancer cell line was obtained from American Type Culture Collection (Manassas, VA, USA)
Summary
In this paper we investigate the effects of potassium hydrogen carbonate and D-ribose in water solution (K:D-rib) on the human breast cancer cell line - Hs 578T. The role of D-ribose in the energetic metabolism [4,5] and its involvement into glycogen synthesis, as well as the importance of K+ into the cell physiology, are well known [6,7]. Agents that stabilize Gquadruplexes can act like anti-tumor agents [10]; so physiological K+ concentration is demanded for not cancer cell behaviour. A feature of many tumors is a change into ions equilibrium, with particular reference to K+ intracellular concentration. Another hallmark in cancer is the reprogrammed chemotaxis pathways in favour of tumor cell dissemination
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
