Abstract
Leukocytes are major cellular components of the inflammatory and immune response systems. After their generation in the bone marrow from hematopoietic stem cells, they maturate as granulocytes (neutrophils, eosinophils, and basophils), monocytes, and lymphocytes. The abnormal accumulation and proliferation of immature blood cells (blasts) lead to severe and widespread diseases such as leukemia. We have recently shown that KCTD15, a member of the potassium channel tetramerization domain containing protein family (KCTD), is remarkably upregulated in leukemic B-cells. Here, we extend our investigation by monitoring the KCTD15 expression levels in circulating lymphocytes, monocytes, and granulocytes, as well as in leukemia cells. Significant differences in the expression level of KCTD15 were detected in normal lymphocytes, monocytes, and granulocytes. Interestingly, we also found overexpression of the protein following leukemic transformation in the case of myeloid cell lineage. Indeed, KCTD15 was found to be upregulated in K562 and NB4 cells, as well as in HL-60 cell lines. This in vitro finding was corroborated by the analysis of KCTD15 mRNA of acute myeloid leukemia (AML) patients reported in the Microarray Innovations in Leukemia (MILE) dataset. Collectively, the present data open interesting perspectives for understanding the maturation process of leukocytes and for the diagnosis/therapy of acute leukemias.
Highlights
White blood cells, denoted as leukocytes, are the major cellular components of the inflammatory and immune response systems that protect against foreign invaders and neoplasia, and they are involved in the repair of damaged tissue [1,2]
A global analysis of the results indicates that KCTD15 is clearly expressed in all of the leukocytes considered here
We showed that KCTD15 was strongly upregulated at mRNA and protein levels in B-acute lymphoblastic leukemia (ALL) samples and cell lines in comparison to peripheral blood mononuclear cells, as well as bone marrow cells after antileukemic therapy
Summary
Denoted as leukocytes, are the major cellular components of the inflammatory and immune response systems that protect against foreign invaders and neoplasia, and they are involved in the repair of damaged tissue [1,2]. Lymphocytes, monocytes, and granulocytes can be effectively identified and distinguished by monitoring the expression level of the CD45 antigen ( defined as leukocyte common antigen, a membrane glycoprotein expressed on almost all hematopoietic cells except for mature erythrocytes) and by analyzing their light-scattering properties using flow cytometry (FCM) [5,6,7]. Additional antigens, such as CD14 and CD33, Diagnostics 2020, 10, 371; doi:10.3390/diagnostics10060371 www.mdpi.com/journal/diagnostics
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