Kaurenoic acid activates TGF-β signaling

Abstract

BackgroundKaurenoic acid (ent-kaur-16-en-19-oic acid: KA) is a key constituent found in the roots of Aralia continentalis Kitagawa (Araliaceae) that has been used for treating rheumatism in traditional Asian medicine. HypothesisAlthough KA was reported to suppress inflammation by activating Nrf2, the anti-inflammatory function of KA is less characterized. Given the complex nature of the inflammatory response and the critical role of TGF-β in resolving inflammation, we hypothesized that KA suppresses inflammatory response by activating TGF-β signaling. MethodsMurine macrophage RAW 264.7, human lung epithelial cell MRC-5, and a TGFβRII defective cell HCT116 were treated with various amounts of KA. KA was also administered to mouse lung via intratracheal (i.t.) route. Phosphorylated Smad2 and Smad3 were analyzed by western blot. TGFβ-dependent gene expression was determined by immunoblotting of α-SMA and luciferase assay. ResultsKA induced the phosphorylation of Smad2 and Smad3, key activator molecules in TGF-β signaling. EW7197, an inhibitor for activin receptor-like kinase 5/TGF-β receptor I (TGFβR1) suppressed KA-mediated phosphorylation of Smad2. Similarly, KA failed to phosphorylate Smad2 in HCT116, suggesting that KA acts through the prototypic TGFβR. KA treatment increased the transcriptional activity driven by a Smad-binding element in a luciferase reporter assay and induced the α-smooth muscle actin (α-SMA). Similarly, i.t. KA induced the phosphorylation of Smad2 and increased the expression ofα-SMA in mouse lungs. ConclusionKA activated TGF-β signaling, suggesting that TGFβ signaling is associated with KA suppressing inflammation.