Abstract
Exercise preconditions against cardiac ischemia reperfusion (IR) injury by activation of the mitochondrial and sarcolemmal ATP sensitive K+ channels (mitoKATP & sarcKATP, respectively), though the extent of protection is unknown. Our purpose was to examine the role of these channels on exercise preconditioning against IR induced apoptosis & necrosis. Male Sprague Dawley rats were treadmill exercised for 3 d, 60 min/d, prior to surgically induced IR injury via coronary artery ligation (LAD)(50min I/120min R). Before surgery, pharmacological inhibitors for mitoKATP (5-Hydroxydecanoate, 5HD) and sarcKATP (HMR-1098) were administered at 10 mg/kg. Hearts were excised and assessed for apoptotic and necrotic biomarkers using TUNEL, TTC, and Western Blotting. The sarcKATP channel was essential to exercise preconditioning against necrosis (p=0.001) while niether the mitoKATP or sarcKATP were essential for antiapoptotic protection. Attenuation of apoptosis could not be explained by the bax:bcl-2 ratio or the cleaved-:total caspase 3 ratio.
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