Abstract
Karyotyping is a technique used to align the chromosomes in the decreasing order of size so that the structural and numerical changes can be easily identified. Traditional chromosome analysis is simplified by the introduction of M_FISH (multiplex fluorescence in-situ hy-bridization), a combinatorial labeling technique in which each chromosomes appears in distinct color. In this paper, Bayes classification of M-FISH chromosome images using the features intensity in five channel as well as size of the chromosome is presented. Watershed transform is employed for segmentation . Also reclassification of small misclassified segments to the neighbor region is performed as post-processing to improve the performance. The classifier was trained and tested on a set of images from the dataset [2] and an overall accuracy of 91.09% was obtained.
Highlights
Chromosomes are microscopic structures found in the cell nuclei that determine the characteristics of an individual
multi-color fluorescence in situ hybridization (M-FISH) is a cytogenic technique developed for the analysis of human chromosomes
Bayes classification of M-FISH chromosome images using the features intensity in five channel as well as size of the chromosome based on watershed segmentation is presented
Summary
Chromosomes are microscopic structures found in the cell nuclei that determine the characteristics of an individual. Chromosomes become visible when they replicate in a process known as mitosis They can be stained and imaged by a microscope during this stage. Many of the genetic problems that cause disorder or disease can be identified by chromosome analysis. Some of the genetic diseases caused by chromosomal abnormalities include autism, down syndrome, mental retardation, etc. M-FISH (multiplex fluorescence in-situ hy-bridization) is a cytogenic technique developed for the analysis of human chromosomes. Unsupervised methods directly classify the M-FISH image without training the classifier. Bayes classification of M-FISH chromosome images using the features intensity in five channel as well as size of the chromosome based on watershed segmentation is presented.
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