Abstract
Karyotypes of six species of Barleria L. occurring in South India viz., B. acuminata, B. buxifolia, B. gibsoni, B. involucrata var. elata, B. noctiflora and B. strigosa are studied. All the six species have the same chromosome number of 2n=40. However, each species is distinct in respect of relative positions of centromere, presence/absence and position of satellites and total haploid chromatin length. The diagnostic value of the karyomorphological details in these taxa is discussed.
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