Abstract
Publisher Summary This chapter presents T cell cloning and hybrid production with the basic approaches and methods required for performing karyotypic analysis on cell lines. Karyotype analysis of T cell clones and hybrids is rapidly becoming a routine part of their characterization. In many cases, such as the monitoring of a cell line over time, examination and counting of solid-stained metaphases is sufficient and requires a minimum amount of time and experience. Evaluation of banding patterns and identification of individual chromosomes requires a greater investment of time and money but brings much more information. The observation that individual murine CTL clones display a large variability in karyotype and karyotypic stability, often including unidentifiable banding patterns, underlines the critical importance of such a detailed analysis. Such changes could have important implications for evaluating data on receptor specificity, genetic rearrangements of cell receptors, and development of the repertoire of antigen specific T cells.
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