Abstract

Detailed somatic karyotype analysis and nuclear DNA estimations in 10 varieties of lentil (Lens culinaris Med.) revealed significant intervarietal variation for chromatin and nuclear DNA contents. The lentil karyotypes were symmetric with preponderance of metacentric chromosomes. Secondary constrictions, located very near to centromere, were observed in one pair of chromosomes each in only 2 varieties, while in the remaining varieties they were not detectable. Correlation analysis revealed a positive linear relationship of 2C DNA amount with total chromosome volume and nuclear area while DNA density has negative relationship with nuclear area. The results indicate that quantitative DNA changes associated with evolution of varieties have affected all chromosomes within the complements but not to the same magnitude.

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