Abstract
Cytological and immunochemical studies were carried out on dioecious Trichosanthes dioica to understand the genetic control of sex expression. The karyotype shows high homogenity and the absence of heteromorphic pair of chromosome in diploid males negates the possibility of XY mechanism. Soluble protein profile from the tuberous roots of the male and female plants of T. dioica did not show any marked distinction; only a variation in the staining pattern was observed. However, in the immunoblot assay the cross reactivity of anti-p-22 (antibody raised against a female biased 22 kD protein of Momordica dioica) at 29 kD and 32 kD regions of the protein profiles in both sex forms establishes p-22 as a sex linked marker. The ratio of the intensity of 29/32 kD polypeptide is also an indicator of sex expression and the data has been analysed as well.
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