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Abstract
Jatropha is an important genus of Euphorbiaceae, with species largely used for various purposes, including the manufacturing of soaps and pharmaceutical products and applications in the bioenergetic industry. Although there have been several studies focusing J. curcas in various aspects, the karyotype features of Jatropha species are poorly known. Therefore, we analyzed six Jatropha species through fluorochrome staining (CMA/DAPI), fluorescent in situ hybridization (FISH) with 5S and 45S rDNA probes and genome size estimation by flow cytometry. Our results revealed several chromosome markers by both CMA/DAPI and FISH for the analyzed species. Five Jatropha species (J. curcas, J. gossypiifolia, J. integerrima, J. multifida and J. podagrica) showed four CMA-positive (CMA+) bands associated with the 5S and 45S rDNA sites (one and two pairs, respectively). However, J. mollissima displayed six CMA+/DAPI- bands co-localized with both 5S and 45S rDNA, which showed a FISH superposition. A gradual variation in the genome sizes was observed (2C = 0.64 to 0.86 pg), although an association between evidenced heterochromatin and genome sizes was not found among species. Except for the unique banding pattern of J. mollissima and the pericentromeric heterochromatin of J. curcas and J. podagrica, our data evidenced relatively conserved karyotypes.
Highlights
Euphorbiaceae is one of the most complex and diverse angiosperm families, presenting a worldwide distribution, mainly in the Americas and in Africa
J. mollissima displayed six CMA+/DAPI- bands co-localized with both 5S and 45S rDNA, which showed a fluorescent in situ hybridization (FISH) superposition
The species J. gossypiifolia and J. podagrica, for instance, have an extensive use as ornamental plants, while J. ribifolia have been used as raw material for soaps and detergents
Summary
Karyotype and genome size comparative analyses among six species of the oilseed-bearing genus Jatropha (Euphorbiaceae). Anne C.T.A. Marinho, Santelmo Vasconcelos , Emanuelle V. Ana Maria Benko-Iseppon and Ana Christina Brasileiro-Vidal1 1Department of Genetics, Universidade Federal de Pernambuco, Recife, PE, Brazil. Ana Maria Benko-Iseppon and Ana Christina Brasileiro-Vidal1 1Department of Genetics, Universidade Federal de Pernambuco, Recife, PE, Brazil. 2Instituto Tecnológico Vale, Belém, PA, Brazil. 3Centro de P&D de Recursos Genéticos Vegetais, Instituto Agronômico, Campinas, SP, Brazil
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