Karyotipe analysis of the tiger barb (Puntigrus tetrazona bleeker, 1855) of North Sumatra

Abstract

The Tiger barb, Puntigrus tetrazona, is an ornamental fish native to Indonesia with a notably striking visual. The research was conducted to perform a karyotype analysis of the Tiger barb chromosomes, including the numbers, sizes, and shapes. The study employed a descriptive approach, by applying 0.007% colchicine for 12 hours. Chromosome preparation was carried out using solid tissue technique, and staining was performed with 5% Giemsa stain. Data analysis was performed using IdeoKar 1.3 software, and the karyotype of the chromosomes was constructed using Adobe Photoshop CS 3. The results of the study showed that the tiger barb had the number of chromosome haploid were 50 with sizes ranging from 1.16 µm to 5.35 µm. The largest value of r was 0.93 and the largest chromosome had a relative length percentage of 5.57. The chromosome shape percentage was 2.54. The highest centromere index obtained 0.47. Furthermore, the study found a TF% value was 40.48%, an Ask% value was 59.51%, an XCI value was 0.38, and an HCL value was 90.08. This research provides an understanding of the karyotype characterization of the tiger barb and an insight for further research in the genetics study of fish.