Karyomorphological delineation and linear differentiation of microsatellite patterns, and meiosis in giant Asian river frog (Limnonectes blyhii) from Thailand

Abstract

The present investigation provides information on linear chromosomal differentiation and microsatellite patterns [d(CA)15, d(CGG)10, d(TA)15] in the genome of giant Asian river frog (Limnonectes blyhii). Five male and female samples each collected from southern Thailand, were used for the study. The metaphase chromosome preparations were prepared from the bone marrow using the standard protocol. Results show that both male and female have the diploid chromosome number 2n = 24, and the fundamental number NF = 48. The karyotypes compose of 4 large metacentric, 4 large submetacentric, 2 medium metacentric, 8 small metacentric and 6 small submetacentric chromosomes. The Ag-NOR, FISH as well as hybridization with the microsatellite d(CGG)10 confirm the location of NOR site in the subcentromeric region on chromosome pair 11. The in situ localization pattern of d(CA)15 microsatellite was positive on chromosome pairs 1 and 6, while microsatellite d(TA)15 show its location in the long arm of chromosome 5,8 and 10. The information provided has value as species specific marker for this species.