Abstract
To increase the number of cytogenetic characters used in Ololygon tripui systematics, we applied some cytogenetic techniques such as Giemsa, C- and NOR-banding, and fluorescence in situ hybridization (FISH) with 18S rDNA and repetitive microsatellite DNA probes to the study of four populations from Minas Gerais State (southeastern Brazil). All populations showed 2n = 24 and FN = 48, and chromosomal formula 8m + 10sm + 6st. Nucleolar organizing regions (NORs) were located on chromosome pair 6 in all populations, although in the Tripuí locality additional markings were observed on one homologue of chromosome pair 3. These patterns were partially congruent with results obtained using the 18S rDNA FISH probe. The microsatellites repetitive DNA (GA)15 and (CAT)10 probes accumulated predominantly in the terminal region of all chromosomes. Chromosome morphology and Ag-NOR were conserved among populations, a conserved pattern in Ololygon Fitzinger, 1843. Repetitive DNA FISH probes patterns were similar among populations, but they revealed species-specific differences when compared with other species of the genus Ololygon, suggesting that molecular cytogenetics are potentially more informative in karyologically conservative taxa.
Highlights
The genus Ololygon Fitzinger, 1843 belongs to Hylidae and currently includes 46 species (Duellman 2016, Frost 2016)
The chromosomal formulae of the O. tripui was 8m + 10sm + 6st, whereas chromosome pair 1, 2, 3, 5, and 8 were sm; 4, 6, and 7 were st, and 9–12 were m in all populations (Figs 1–4)
The diploid number of 2n = 24 and FN = 48 observed in O. tripui was similar to those reported for all studied species of the genus and considered as a highly conserved character, which is shared with other genera within Hylidae (i.e. Scinax, Xenohyla Izecksohn, 1998, and Lysapsus Cope, 1862) (Suárez et al 2013)
Summary
The genus Ololygon Fitzinger, 1843 belongs to Hylidae and currently includes 46 species (Duellman 2016, Frost 2016). Karyotypes of Ololygon and Scinax Wagler, 1830 are highly conserved: all species show 2n = 24, and FN = 48, and morphological differences are restricted to slight variations between corresponding homeologous chromosomes (Nunes and Fagundes 2008, Cardozo et al 2011, Nogueira et al 2015, Peixoto et al 2015). Within Ololygon, only 19 recognized species and four unnamed taxa have been karyologically studied. The species from the genus Ololygon were considered as Scinax (Frost 2016)
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