Abstract
India). Karyological analysis of cultured cells and regenerated plants of Cipura paludosa Aubl. (Iridaceae)-A structural hybrid. Bull. Torrey Bot. Club I 1 5: 280-289. 1988.-Cipurapaludosa, a structural hybrid of the Iridaceae with a heteromorphic pair of long chromosomes, has been investigated in vitro. Propagation as well as regeneration of plantlets from callus culture of quartered bulb scale explants has been achieved successfully on MS medium supplemented with specific growth substances. Calluses revealed a high frequency of diploid mitoses from initiation to 40-week-old cultures, though hyperdiploid and hypertetraploid cells were noted as well. Small chromosomes were more susceptible to numerical alteration, while the long heteromorphic pair of chromosomes was stable. Regenerated plants revealed a relative karyotypic stability. Almost 80% of the plants were diploid with a parental (2Asm-st + 2Bm + 8Cmnm) karyotype. The remaining 20% of the plants were mosaics, with a diploid mode number. Structural alteration of chromosomes was very rare in regenerants but confined to the long chromosomes only. The expected cytological behavior of a structural hybrid in vitro is discussed.
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