KARBOPLATİNE BAĞLI ORGAN TOKSİSİTELFRİNDE PENTOKSİFİLİN, C VİTAMİNİ VE E VİTAMİNİNİN ETKİSİ

Abstract

In this study, the protective effects of pentoxifylline (PTX), vitamin C and vitamin E on carboplatin toxicity were comparatively investigated in different organs and tissues in rats. Thirty adult rats were divided into 5 groups (n=6), each comprising six animals and the groups were treated daily for five days, by i.p. injections of 0, 2 mL saline (Group I), 25 mg/kg carboplatin (Group II), 25 mg/kg carboplatin + 150mg/kg pentoxifylline (Group III), 25 mg/kg carboplatin + 50 mg/kg vitamin C (Group IV) and 25 mg/kg carboplatin + 50 mg/kg vitamin E (Group V). At the end of the treatment, blood samples were collected by direct cardiac puncture and serum was used for the assay of rnarker enzymes, alanine aminotransferase (ALT), alkaline phosphatase (ALP), direct and indirect bilirubin. Tissue samples were taken and processed by Standard histological techniques. At the end of the study, while there were no pathological alterations in the carboplatin group, ALT and ALP activities were significantiy increased compared to the control group. There were also no pathological signs in the pentoxifylline, vitamin C and vitamin E groups and ALT, ALP and indirect bilirubin levels were not different from control, but significantiy lower than carbopiatin groups. These results indicate that the combination of carboplatin with the vitamin C, vitamin E or pentoxifylline may protect against hepatotoxicity.