Kanserli hücre hatları, pasaj sayısı arttıkça genomik organizasyonunu ve karyotipini değiştirir: sitogenetik bir çalışma

Abstract

Purpose: The limited use of mammals in human health related scientific research has led to the development of new research strategies like cell culture techniques. Commercially available cancerous cell lines that are well characterized by cytogenetics and biochemical markers allow comparison of results among different laboratories. However, as these cell lines tend to be maintained in culture over long periods of time, mutations can occur that may change characteristics and responses of cell lines that have initially been identified or non-existed at earlier passages. Here we cytogenetically investigated the chromosomal rearrangements in repeated cultures of six different cell lines over continuous passages. Method: MCF7, HCT116, A549, SHSY5Y, HEPG2, and NIH3T3 cell lines were cultured in DMEM containing 10% FBS and 1% penicillin-streptomycin. GTG banding procedure was used for the analysis of metaphase chromosomes, at least 20 metaphases were analyzed per cell line. Results: We found chromosome number variations and structural changes in the all examined cell cultures as the passage numbers increase. Conclusion: Cell lines have long been used in research to test drugs, to delineate molecular mechanisms, to understand the environmental effects and so on. The most important feature of a cell line is its genotype and karyotype similarities with their host organism. Cancer Cell lines, possess genomic/chromosomal instability that also lead them to change their phenotype along with their karyotype from one passage to next. Therefore, it is always best to verify karyotype before employing a specific cell line in a research project.