Abstract
Background Kangquan Recipe (KQR) is a traditional Chinese medicine compound made by our research group for the treatment of benign prostatic hyperplasia (BPH). Whether KQR can treat BPH as a single drug or play a role in the treatment of BPH in combination therapy needs further study. Aim of the Study To investigate the effect of KQR on the expression of TGF-β/Smad signaling pathway-related factors in rats with BPH. In-depth analysis revealed the relevant signal transduction mechanism by which KQR acts to treat BPH. Materials and Methods Forty-eight male Sprague-Dawley rats were randomly divided into six groups of 8 rats each. In addition to the control group, 40 rats were castrated and then injected with testosterone propionate to form a prostatic hyperplasia model. After 30 days, three groups received different concentrations of KQR (14 g/kg, 7 g/kg, and 3.5 g/kg), and the finasteride group received 0.5 mg/kg finasteride. The BPH group and the control group received the same volume of saline. All groups were treated for a total of 30 days. Rat body weight, prostate volume, wet weight, index, histology, and the mRNA and protein levels of TGF-β, TGF-βR1, TGF-βR2, p-Smad2, p-Smad3, BAMBI, E-cadherin, and N-cadherin in the prostate tissue were measured after the end of treatment. Results Compared with the control group, the BPH group had increased prostate wet weight, volume, and index, and the histology showed significant BPH. Compared with the BPH group, the three KQR groups and the finasteride group all had varying levels of reduction in the prostate wet weight, volume, and index of the prostate and varying degrees of improvement in the histological manifestations of BPH. KQR downregulates the mRNA and/or protein expression of TGF-β, TGF-βR1, TGF-βR2, p-Smad2, p-Smad3, and N-cadherin protein in prostate tissue and increases the mRNA and protein expression of BAMBI and E-cadherin protein. Conclusions In the model of BPH induced by testosterone propionate after castration, KQR can inhibit the conduction of the TGF-β/Smad signaling pathway by upregulating the expression of BAMBI protein and reversing EMT in rat prostate tissue.
Highlights
Benign prostatic hyperplasia (BPH) is a common disease in elderly men, with lower urinary tract symptoms (LUTS) caused by hyperplasia of the prostatic epithelium and stromal cells [1]
Compared with the finasteride group, the Kangquan Recipe (KQR) high and middle dose groups had no significant differences in prostate wet weight, prostate volume, and Prostate index HE (PI) values (P>0.05)
KQR could significantly reduce the prostate wet weight, prostate volume and PI of rats with benign prostatic hyperplasia (BPH); its effect had a positive relationship with the drug concentration
Summary
Benign prostatic hyperplasia (BPH) is a common disease in elderly men, with lower urinary tract symptoms (LUTS) caused by hyperplasia of the prostatic epithelium and stromal cells [1]. The main therapeutic drugs for BPH are 5α-reductase inhibitors and α-blockers [2, 3], such as finasteride and terazosin They can cause adverse reactions such as fatigue, hypotension, ejaculation disorders, sexual dysfunction, and an increased risk of prostate fibrosis [3, 4]. Prostate volume, wet weight, index, histology, and the mRNA and protein levels of TGF-β, TGF-βR1, TGF-βR2, p-Smad, p-Smad, BAMBI, E-cadherin, and N-cadherin in the prostate tissue were measured after the end of treatment. In the model of BPH induced by testosterone propionate after castration, KQR can inhibit the conduction of the TGF-β/Smad signaling pathway by upregulating the expression of BAMBI protein and reversing EMT in rat prostate tissue
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