Kangiella geojedonensis sp. nov., isolated from seawater

Abstract

A Gram-stain-negative, non-motile, non-spore-forming bacterial strain, YCS-5(T), was isolated from seawater off the southern coast of Korea. Strain YCS-5(T) grew optimally at 30 °C and in the presence of 2% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain YCS-5(T) fell within the clade comprising Kangiella species. Strain YCS-5(T) exhibited 16S rRNA gene sequence similarity values of 96.6, 95.7 and 97.9% to the type strains of Kangiella koreensis, Kangiella aquimarina and Kangiella japonica, respectively, and less than 89.8% to strains of other species used in the phylogenetic analysis. Strain YCS-5(T) contained Q-8 as the predominant ubiquinone and iso-C(17:0), iso-C(15:0), iso-C(11:0) 3-OH and iso-C(17:1)ω9c as the major fatty acids. The polar lipid profile of strain YCS-5(T) was similar to that of K. koreensis SW-125(T), with phosphatidylglycerol and an unidentified aminolipid as major polar lipids. The DNA G+C content was 47 mol%. The mean DNA-DNA relatedness value between strain YCS-5(T) and K. japonica JCM 16211(T) was 12%. Differential phenotypic properties and the phylogenetic and genetic distinctiveness of strain YCS-5(T) demonstrated that this strain is distinguishable from other Kangiella species. On the basis of the data presented, strain YCS-5(T) is considered to represent a novel species of the genus Kangiella, for which the name Kangiella geojedonensis sp. nov. is proposed; the type strain is YCS-5(T) (=KCTC 23420(T)=CCUG 60526(T)).