Abstract

Cantaloupe (Cucumis melo var. Cantalupensis L.), a fruit containing some compounds, have activity to protect human body from free radical. The beta carotene (provitamin A) was recognized as antioxidant compound. Antioxidants could protect the body from cardiovascular damage caused by free radical. The aims of this study was to determine the levels of beta carotene and antioxidant activity (IC 50 ) from Cucumis melo var. cantalupensis L. extract using DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Cantaloupe was extracted using n-hexan-acetone-methanol ratio (2: 1: 1). Identification of beta carotene was performed using 25% antimony trichloride reagent followed by TLC and UV-Vis spectrum test compared with beta carotene as standard. The content of beta carotene and free radical scavenging activity was measured spectrophotometrically at 425 nm. The beta carotene level of cantaloupe was 3.171 ± 0.150 %. The antioxidant activity, presented as IC 50 value of cantaloupe extract, was 12.137±0.44 µg/mL and the antioxidant activity of standard beta carotene was 2.15 ± 0.172 µg/mL. The cantaloupe extract contained beta carotene compunds but the antioxidant activity of extract was lower than beta carotene standard.

Highlights

  • Antioxidants could protect the body from cardiovascular damage caused by free radical

  • The aims of this study was to determine the levels of beta carotene

  • 25% antimony trichloride reagent followed by TLC and UV-Vis spectrum test compared with beta carotene

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Summary

Introduction

Buah ini mengandung beta karoten (provitamin A) yang berfungsi sebagai antioksidan. Penelitian ini bertujuan mengetahui kadar beta karoten dan mengetahui aktivitas antioksidan (IC50) ekstrak blewah Kadar beta karoten dan aktivitas penangkapan radikal bebas diuji metode spektrofotometri pada 425 nm. Antioksidan dapat melawan pengaruh bahaya dari radikal bebas sebagai hasil metabolisme oksidatif, yaitu hasil reaksi-reaksi kimia dan proses metabolik yang terjadi di dalam tubuh.

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