Abstract
In this study, we present a highly selective and sensitive technique for the detection of DNA using kanamycin A-functionalized AuNPs and ethidium bromide (a dye that binds specifically to DNA). Here, we demonstrate the interaction of the kanamycin A-modified-citrate gold nano particles with the DNA. Also, the interaction of the ethidium bromide-DNA complex with the kanamycin A-modified AuNPs is shown. Florescence is detected whenever the EtBr-DNA complex interacts with the kanamycin A-modified AuNPs. The transmission electron microscopic studies were also done to show the aggregation of the modified AuNPs in presence of the DNA. This further indicates that the binding of the kanamycin A modified gold particles to the DNA. This assay can be applicable in large scale characterization of DNA interactions and may also become the choice to complement conventional techniques in the non viral gene delivery system.
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