Abstract
The aim of this study was to investigate whether pretreatment with the Japanese herbal medicine "Hochu-ekki-to" (TJ-41) has an ameliorative effect on carbon tetrachloride (CCl4)-induced hepatotoxicity through anorexia prevention. Twenty-four hours before CCl4 injection, TJ-41 or saline solution was intraperitoneally administered. Furthermore, 24h after TJ-41 injection, mice were intraperitoneally administered 1.6g/kg CCl4 or olive oil. Moreover, 24h after CCl4/olive oil injection, mice from each group were euthanized and bled for plasma analysis. Mice injected with CCl4 exhibited severe anorexia. Moreover, CCl4 increased the plasma levels of hepatic injury markers (i.e., alanine aminotransferase and aspartate aminotransferase) as well as lipid peroxidation and hepatic Ca levels. Pretreatment with TJ-41 recovered the CCl4-induced anorexia and plasma levels of the hepatic injury markers. Moreover, CCl4-induced lipid peroxidation and hepatic Ca levels decreased upon TJ-41 pretreatment. In addition, hepatic metallothionein levels in the TJ-41+CCl4-treated group were decreased by >50% compared with the levels in the TJ-41-treated group, implying that metallothionein was consumed by CCl4-induced radicals. Our results suggest that TJ-41 attenuates CCl4-induced hepatotoxicity, presumably by the induction of metallothionein, which in turn scavenges radicals induced by CCl4 exposure.
Highlights
The liver is one of the most important organs in the human body and has multiple functions such as detoxification, protein synthesis, and production of chemicals necessary for digestion
Objective The aim of this study was to investigate whether pretreatment with the Japanese herbal medicine ‘‘Hochuekki-to’’ (TJ-41) has an ameliorative effect on carbon tetrachloride (CCl4)-induced hepatotoxicity through anorexia prevention
CCl4-induced lipid peroxidation and hepatic Ca levels decreased upon TJ-41 pretreatment
Summary
Mice injected with CCl4 exhibited severe anorexia. CCl4 increased the plasma levels of hepatic injury markers (i.e., alanine aminotransferase and aspartate aminotransferase) as well as lipid peroxidation and hepatic Ca levels. Pretreatment with TJ-41 recovered the CCl4induced anorexia and plasma levels of the hepatic injury markers. CCl4-induced lipid peroxidation and hepatic Ca levels decreased upon TJ-41 pretreatment. Hepatic metallothionein levels in the TJ41 ? CCl4-treated group were decreased by [50 % compared with the levels in the TJ-41-treated group, implying that metallothionein was consumed by CCl4-induced radicals Hepatic metallothionein levels in the TJ41 ? CCl4-treated group were decreased by [50 % compared with the levels in the TJ-41-treated group, implying that metallothionein was consumed by CCl4-induced radicals
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