Abstract
Red durian plants have the potential to be developed because they have high economic value. Red durian plants are propagated conventionally in two ways, namely through seeds and shoot grafts. Propagation using shoot graft creates a new problem for red durian farmers, namely a decrease in fruit production on the parent plant due to large amounts of scion. One alternative to the propagation of red durian seeds without damaging the parent plant can be done by using tissue culture techniques. The explants used in this study was durian callus. The callus was grown on B5 media treated with a plant growth regulator combination in the form of TDZ consisting of six treatments, namely 0, 0.4, 0.8, 1.2, 1.6 ppm and BA consisting of five treatment, namely 0, 0.3, 0.6, 0.9, 1.2 ppm. This research was conducted to determine the best dose of combination treatment of tidiazuron (TDZ) with BA in the formation of shoots from red durian callus through tissue culture. The results showed that at the age of 6 months after culture, red durian callus had a color change from yellow to green in the TDZ treatment 1.6 ppm + BA 1.2 ppm.
Highlights
Sedangkan perbanyakan menggunakan sambung pucuk menimbulkan permasalahan baru bagi petani durian merah yaitu terjadi penurunan produksi buah pada tanaman induk akibat pengambilan entres dalam jumlah besar
Pengaruh Variasi Konsentrasi NAA dan BAP tehadap Induksi Kalus Jarak Pagar
Summary
Penelitian ini dilakukan di laboratorium kultur jaringan fakultas pertanian dan perikanan universitas 17 agustus 1945 Banyuwangi pada bulan Maret – November 2020. Selanjutnya di dalam media tersebut ditambahkan kombinasi perlakuan zat pengatur tumbuh TDZ dan BA. HASIL DAN PEMBAHASAN Jenis Kontaminan pada Media dengan Kombinasi Perlakuan TDZ dan BA. Hasil pengamatan secara visual menunjukkan bahwa kalus setelah kultur dengan tingkat kontaminasi durian merah yang ditumbuhkan pada. Tingkat Kontaminasi Eksplan (Kalus Durian Merah) pada Perlakuan kombinasi TDZ dengan BA. Menunjukkan bahwa Jenis kontaminan yang muncul pada media kultur jaringan kalus durian merah dengan kombinasi perlakuan TDZ dan BA adalah jamur. Jenis Kontaminan eksplan (kalus durian merah) pada Perlakuan kombinasi TDZ dengan BA. Dkk (2014), berpendapat bahwa kontaminan ini jika tidak dihilangkan maka akan hidup dan berkembang di dalam kultur sehingga dapat menghambat, bahkan menyebabkan kematian eksplan. Pengaruh Kombinasi TDZ dengan BA Terhadap Warna Kalus Durian Merah
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