Abstract
The immunofluorescence of antibody-labelled serotonin was used as an indicator for the amount of intracellular serotonin. Incubation in Ringer solution containing serotonin and pargyline increased the immunofluorescence which was subsequently decreased in a calcium-dependent manner by incubation in high-potassium Ringer. If preloaded retinas were exposed to 10 μM kainic acid, the immunofluorescence decreased in a time-dependent way. This kainic acid-induced release was blocked by piperidine dicar☐ylic acid. These results support the idea that serotonin is a transmitter in OFF-bipolar cells.
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