Abstract
Model organism studies are widely implemented in biomedical research fields. Zebrafish is a common and convenient model organism. To provide in vivo investigation of living zebrafish the non-invasive imaging methods are implemented. Hyperspectral imaging utilizing acousto-optic tunable filters is a perspective modality for zebrafish embryos and larvae automated observation. In this paper, the hyperspectral microscope based on the acousto-optical tunable filter is described. Using the hyperspectral image arrays obtained with the described setup, the K-means clustering algorithm is tested. The results obtained for different number of clusters are presented and discussed.
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