K Fertilizers Reduce the Accumulation of Cd in Panax notoginseng (Burk.) F.H. by Improving the Quality of the Microbial Community.

Yue Shi,Jinhui Man,Lanping Guo,Chunyan Dai,Rongfeng Pu,Ye Yang,Xiuming Cui,Lisha Qiu,Bingpeng Shang,Xiaohong Ou,Pengfei Liu

doi:10.3389/fpls.2020.00888

Abstract

The high background value of cadmium (Cd) in the Panax notoginseng planting soil is the main reason for the Cd content in P. notoginseng exceeding the limit standards. The main goal of this study was to reveal the mechanism by which potassium (K) reduces Cd accumulation in P. notoginseng from the perspective of the influences of soil microbial communities on soil pH, total organic matter (TOM) and cation exchange capacity (CEC). Pot experiments were conducted to study the effects of different types and amounts of applied K on the Cd content in P. notoginseng, and on the soil pH, TOM, CEC, and bioavailable Cd (bio-Cd) content in soil. Field experiments were conducted to study the effects of K2SO4 fertilizer on the microbial community, and its correlations with the soil pH, TOM and CEC were analyzed. A moderate application of K2SO4 (0.6 g⋅kg–1) was found to be the most optimal treatment for the reduction of Cd in the pot experiments. The field experiments proved that K fertilizer (K2SO4) alleviated the decreases in pH, TOM and CEC, and reduced the content of bio-Cd in the soil. The application of K fertilizer inhibited the growth of Acidobacteria, but the abundances of Mortierellomycota, Proteobacteria and Bacteroidetes were promoted. The relative abundances of Acidobacteria and Proteobacteria in the soil bacteria exhibited significant negative and positive correlations with pH and CEC, respectively. In contrast, the relative abundance of Mortierellomycota was found to be positively correlated with the pH, TOM and CEC. The bio-Cd content was also found to be positively correlated with the relative abundance of Acidobacteriia but negatively correlated with the relative abundances of Proteobacteria and Mortierellomycota. The application of K fertilizer inhibited the abundance of Acidobacteria, which alleviated the acidification of the soil pH and CEC, and promoted increase in the abundances of Mortierellomycota, Proteobacteria and Bacteroidetes, which ultimately increased the soil TOM and CEC. Soil microorganisms were found to mitigated decreases in the soil pH, TOM, and CEC and reduced the bio-Cd content in the soil, which significantly reduced the accumulation of Cd in P. notoginseng.

Highlights

The genuine producing area of Panax notoginseng (Burk.) F
The bioavailable Cd (bio-Cd) content represents the portion of Cd in the soil that can be absorbed and utilized by plant
The pH, total organic matter (TOM), and cation exchange capacity (CEC) have been identified as the main factors that affect the bioavailability of heavy metals in soil (Li and Song, 2003; Liang et al, 2013), and can be significantly regulated by fertilization

Summary

Introduction

The genuine producing area of Panax notoginseng (Burk.) F. H. is Yunnan Province, China (Yang et al, 2018), which generates approximately 98% of the P. notoginseng medicinal materials on the Chinese market (Liu, 2019). Yunnan Province accounts for 46% of China’s Cd production (Liu et al, 2016). Its resulted in exceeding the standard rate by 35% and 23% of P. notoginseng planting soils and medicinal material, respectively (Ou et al, 2016; Shi et al, 2019). The ability to protect P. notoginseng from Cd has drawn considerable attention from consumers and regulatory departments (Lin et al, 2014). There is a need to develop a low-cost and high-efficiency Cdblocking technology for P. notoginseng as well as to elucidate the underlying mechanisms by which Cd can be blocked

Objectives

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Results

Conclusion