Abstract
Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disorder. Traumatic stress during adolescence increases the risk of IBS in adults. The aim of this study was to characterize the juvenile social defeat stress (SDS)-associated IBS model in mice. Juvenile mice were exposed to an aggressor mouse for 10 min once daily for 10 consecutive days. Behavioral tests, visceral sensitivity, immune responses, and fecal bacteria in the colon were evaluated in 5 weeks after SDS exposure. Social avoidance, anxiety- and depression-like behavior, and visceral hypersensitivity were observed. Juvenile SDS exposure significantly increased the number of 5-HT-containing cells and calcitonin gene-related peptide-positive neurons in the colon. The gut microbiota was largely similar between the control and juvenile SDS groups. The alterations in fecal pellet output, bead expulsion time, plasma corticosterone concentration, and colonic 5-HT content in response to restraint stress were exacerbated in the juvenile SDS group compared with the control group. The combination of juvenile SDS and restraint stress increased the noradrenaline metabolite 3-Methoxy-4-hydroxyphenylglycol (MHPG) content and MHPG/noradrenaline ratio in the amygdala when compared with restraint stress in control mice. These results suggest that juvenile SDS exposure results in later onset of IBS-like symptoms.
Highlights
Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disorder
There are multiple ways to measure the outcome in an experimental animal model of IBS, including physiologic and reflex responses, spontaneous behaviors, visceral pain-directed complex behaviors, or brain responses[19]
We confirmed that juvenile social defeat stress (SDS) caused anxiety- and depression-like behaviors and visceral hypersensitivity in adult mice
Summary
Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disorder. The combination of juvenile SDS and restraint stress increased the noradrenaline metabolite 3-Methoxy-4-hydroxyphenylglycol (MHPG) content and MHPG/noradrenaline ratio in the amygdala when compared with restraint stress in control mice These results suggest that juvenile SDS exposure results in later onset of IBS-like symptoms. In which pups are separated for 3 h daily during early postnatal days, is a form of trauma due to maternal neglect This stress induces long-lasting IBS-like alterations in rats, such as visceral hypersensitivity, enhanced intestinal motility in Scientific Reports | (2021) 11:16276. Exposing juvenile mice to SDS may be useful for studying the pathogenesis of chronic stress-related psychiatric impairments in adolescents with early adverse experiences
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