Abstract
1. 1. A radiochemical assay was used to examine juvenile hormone (JH) synthesis and secretion in vitro by incubating two pairs of larval corpus cardiacum-corpus allatum complexes (CC-CA) from, Lymantria dispar, in 50 μl of osmotically balanced Grace's medium containing 1 μC1 [ 3H-methyl]-methionine for 6 hr. 2. 2. For CC-CA of fourth instar female larvae, maximal incorporation of 3H-methyl was 0.15 pmol/pr/hr between days 2 and 3. High pressure liquid chromatographic (HPLC) analysis suggested that the biosynthetic products are mainly JH III with a little JH II at times. 3. 3. For CC-CA of last instar female larvae, incorporation of 3H-methyl was 0.48 pmol/pr/hr at the beginning of the stadium and decreased to negligible levels by day 10. HPLC analysis suggested that CC-CA of last instar larvae produced only JH III. Volume increases in CA during the last instar were associated with declining activities of JH secretion. 4. 4. Comparisons of maximal rates of 3 H-methyl incorporation by each unit volume of CA revealed that in the last instar each unit volume (μm 3) of glandular tissue secreted 50% more JH than in the fourth instar.
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