Abstract
In this article we introduce JULIDE, a software toolkit developed to perform the 3D reconstruction, intensity normalization, volume standardization by 3D image registration and voxel-wise statistical analysis of autoradiographs of mouse brain sections. This software tool has been developed in the open-source ITK software framework and is freely available under a GPL license. The article presents the complete image processing chain from raw data acquisition to 3D statistical group analysis. Results of the group comparison in the context of a study on spatial learning are shown as an illustration of the data that can be obtained with this tool.
Highlights
Functional brain imaging techniques such as Positron Emission Tomography (PET) and Magnetic Resonance (MR) have revolutionized the study of brain function in humans
We present a new software tool, called JULIDE, that we developed to perform the 3D reconstruction, intensity normalization, volume standardization by 3D image registration and voxel-wise statistical analysis of autoradiographs of mice
Statistical comparison between groups can be made with JULIDE: a voxel-wise student t-test is performed between the data of two groups, to identify the regions with significantly different activation
Summary
Functional brain imaging techniques such as Positron Emission Tomography (PET) and Magnetic Resonance (MR) have revolutionized the study of brain function in humans. We present a new software tool, called JULIDE, that we developed to perform the 3D reconstruction, intensity normalization, volume standardization by 3D image registration and voxel-wise statistical analysis of autoradiographs of mice.
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