Abstract

This study aims to characterize the proximate composition, antioxidant activity, phytochemical profile, anti-diabetic, and anti-inflammatory properties of Juglans regia kernel meal (JKM). The examination of the proximate composition reveals that JKM contains moisture (7.74%), ash (4.46%), crude fat (31.26%), crude fiber (8.41%), crude protein (8.99%) and nitrogen-free extract (39.14%). The analysis of JKM for antioxidant properties shows lipid peroxidation inhibition (63.78%), ferric ion reducing antioxidant power (103.44 mg/g), 2,2-diphenyl-1-picrylhydrazyl hydrate (57.91%), and vitamin C (152.87 mg/g). The phytochemical compositional analysis shows that JKM has alkaloids (12.08 %), saponins (43.49 mg/g), steroids (4.84 mg/g), flavonoids (14.74 mg/g), tannins (1.69 mg/g) and phenol (35.93 mg/g). The JKM also demonstrated alpha-amylase inhibition and alpha-glucosidase inhibition activities of 61.06% and 67.76%, respectively; while 62.71% and 79.17% were reported for the albumin denaturation inhibition and antiproteinase activity of JKM, respectively. JKM dietary supplementation may enhance the animals' welfare. It is advised to employ it in an animal model, though.

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