Journey beyond the embryo: The beauty of Pristina and naidine annelids for studying regeneration and agametic reproduction.

Abstract

Regeneration, asexual agametic reproduction, and other forms of postembryonic development are ecologically important and widely variable across animals, yet our understanding of this important aspect of animal diversity remains limited. A significant limitation has been the dearth of adequate study systems for exploring the mechanisms and evolution of these processes. Here I describe key parts of our journey in developing naid annelids as a study system for investigating the evolution and development of regeneration and fission. Naids are small freshwater annelids that provide numerous advantages for studying postembryonic development: they are small and reproduce readily by fission, they include species with diverse regenerative abilities, and many species are easy to culture. Among the naids, Pristina leidyi is a particularly useful study species, being largely transparent and quite robust to a variety of experimental manipulations. Building on a sparse but long history of past research on these animals, we have developed this system by establishing methods and generating resources for working with them. Naids are yielding novel insights into the evolution of regeneration and fission, providing one of many examples of the value of developing new study species to enable the exploration of fundamental and understudied questions in biology. Establishing new study systems comes with challenges but is exciting and rewarding, and I provide perspectives from my own experiences with the hope of encouraging the further expansion of study systems in biology.