Abstract
Polymorphic isozymes visualized by starch gel electrophoresis and histochemical staining were examined in parents and 1,000 progeny of 24 single-pair matings of channel catfish Ictalurus punctatus and three interspecific crosses of channel catfish × blue catfish I. furcatus. Polymorphic loci that could be scored from fin tissue included aconitate hydratase (mAH*), glucose-6-phosphate isomerase (GPI-B*), glutathione reductase (GR*), phosphoglucomutase (PGM*), and UDPglucose-hexose-1-phosphate uridylyltransferase (UGHUT-2*). Inheritance of observed electrophoretic variation was consistent with Mendelian segregation of codominant alleles in progeny selected randomly from the individual crosses. Although mAH*, GPI-B*, PGM*, and UGHUT-2* appeared to assort independently within the limits of the data, strong evidence for linkage of GR* and PGM* was obtained (19.7% recombination, P ≤ 0.001 for data combined from two informative pedigrees); this linkage was designated Ictalurus punctatus linkage group I. This study represented a first step in assembly of a catfish gene map large enough to provide genetic markers for each catfish chromosome to enable detection of specific loci associated with traits of economic importance by genetic linkage analyses. For the polymorphic loci studied here, no consistent association of genotype with average body weight was observed.
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