Joint effects of gamma radiation and zinc on duckweed Lemna minor L.

Abstract

When assessing the consequences of combined chemical and radiation pollution on bodies of water, it is important to take into account the interaction of different factors, especially the possible synergistic increase in the toxic effect on growth, biochemical and physiological processes of living organisms. In this work, we studied the combined effect of γ-radiation and zinc on freshwater duckweed Lemna minor L. Irradiated plants (doses were 18, 42, and 63 Gy) were placed on a medium with an excess of zinc (3.15, 6.3, 12.6 μmol/L) for 7 days. Our results showed that the accumulation of zinc in tissues increased in irradiated plants when compared to non-irradiated plants. The interaction of factors in assessing their effect on the growth rate of plants was most often additive, but there was also a synergistic increase in the toxic effect at a zinc concentration of 12.6 μmol/L and irradiation at doses of 42 and 63 Gy. When comparing the combined and separate effects of gamma radiation and zinc, it was found that a reduction in the area of fronds (leaf-like plates) was caused exclusively due to the effects of radiation. Zinc and γ-radiation contributed to the enhancement of membrane lipid peroxidation. Irradiation stimulated the production of chlorophylls a and b, as well as carotenoids.